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phoR [2019-04-14 15:19:18]
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phoR [2019-04-14 15:19:18]

two-component sensor kinase, regulation of phosphate metabolism
locus
BSU29100
pI
5.96
mw
64.95 kDa
protein length
579 aa Sequence Blast
gene length
1740 bp Sequence Blast
function
regulation of phosphate metabolism
product
two-component sensor kinase
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.3|Phosphate metabolism]
  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.2|Protein kinases]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.2|Control of two-component response regulators] → [category|SW 3.4.2.2.1|Two-component sensor kinase]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.1|General stress proteins (controlled by SigB)]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.4|Phosphorylation on a His residue]

    • Gene

    Coordinates
    2,976,068 → 2,977,807

    The protein

    Catalyzed reaction/ biological activity

  • autophosphorylation, phosphorylation of [protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP]

    • Paralogous protein(s)

  • [protein|E88978809272FAC2520DB4ABCA0554F8028F3451|WalK], [protein|EA6790EF30D3DDB9670FE52DFD2C5083AB6A48E1|ResE]

    • [SW|Domains]

  • two transmembrane segments
  • [SW|PAS domain], for binding of an intermediate of wall teichoic acid biosynthesis (lipid V composed of poly(glycerol phosphate)) [Pubmed|25315493]
  • C-terminal histidine phosphotransferase domain

    • Modification

  • autophosphorylation on a His residue in response to the the availability of an intermediate of wall teichoic acid bioynthesis, autophosphorylation is prevented by binding of this intermediate to the intracellular [SW|PAS domain] of [protein|C81F68521F17861D50926D7D8757DC1D2340BC0D|PhoR] [Pubmed|25315493]

    • Effectors of protein activity

  • activity is inhibited by binding of an intermediate of wall teichoic acid biosynthesis (lipid V composed of poly(glycerol phosphate) to the intracellular [SW|PAS domain] of [protein|C81F68521F17861D50926D7D8757DC1D2340BC0D|PhoR] [Pubmed|25315493]
  • the kinase activity is activated by an intermediate in the WTA biosynthetic pathway (product of [protein|72A89412C53703F96F835D26307263F5D1A4AB4E|TagA] or [protein|911A4D957A6C0DFC636A152395119DBFC777DDA2|TagB]) [pubmed|29644746]

    • Structure

  • [PDB|3CWF] (extracellular domain) [Pubmed|20008068]

    • Expression and Regulation

    Operons

    genes
    [gene|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|phoP]-[gene|C81F68521F17861D50926D7D8757DC1D2340BC0D|phoR]
    description
    [Pubmed|14762014]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|16452408], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [Pubmed|15205429], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]
  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, [Pubmed|15699190,15205429], in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]

    • regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|16452408,12850135], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]
  • [protein|F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|ScoC]: repression, [Pubmed|20382764], in [regulon|F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|ScoC regulon]
  • [protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP]: activation, due to [protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP]~P binding to the 3’end of the [protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP]-[protein|C81F68521F17861D50926D7D8757DC1D2340BC0D|PhoR] operon [Pubmed|25666134,15205429,14762014], in [regulon|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP regulon]

    • regulation

  • carbon catabolite repression ([protein|search|CcpA]) [Pubmed|16452408,12850135]
    • view in new tab

    Biological materials

    Mutant

  • 1A966 ( ''phoR''::''tet''), [Pubmed|14973033], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A966&Search=1A966 BGSC]
  • BKE29100 (Δ[gene|C81F68521F17861D50926D7D8757DC1D2340BC0D|phoR]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE29100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TACAGAAAAAAGGCGCACAC, downstream forward: _UP4_TAATGTTTACAAAGGTTTAA
  • BKK29100 (Δ[gene|C81F68521F17861D50926D7D8757DC1D2340BC0D|phoR]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK29100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TACAGAAAAAAGGCGCACAC, downstream forward: _UP4_TAATGTTTACAAAGGTTTAA

    • labs

  • [SW|Marion Hulett], University of Illinois at Chicago, USA [http://www.uic.edu/depts/bios/faculty/hulett/hulettm.shtml Homepage]

    • References

    Reviews

  • 10094672,25355628,30459743

    • Original publications

  • 20008068,10433720,15205429,9084179,17085571,9987123,10913081,16452408,20167622,15205429,14762014,20382764,25315493,29644746